tool to design flanking oligo nucleotides for DNA amplification
DescriptionPrimer3 picks primers for Polymerase Chain Reactions (PCRs), considering as criteria oligonucleotide melting temperature, size, GC content and primer-dimer possibilities, PCR product size, positional constraints within the source sequence, and miscellaneous other constraints. All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.
1158 other people were interested in this package here. The newest known version of this software is 2.4.0-2 (Information last updated about 21 hours ago.)
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