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primer3

tool to design flanking oligo nucleotides for DNA amplification

tool to design flanking oligo nucleotides for DNA amplification
from version 2.3.6-1

Description

Primer3 picks primers for Polymerase Chain Reactions (PCRs), considering as criteria oligonucleotide melting temperature, size, GC content and primer-dimer possibilities, PCR product size, positional constraints within the source sequence, and miscellaneous other constraints. All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.

Homepage

http://primer3.sourceforge.net


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