primer3-examples

tool to design flanking oligo nucleotides for DNA amplification (examples)

Description

Primer3 picks primers for Polymerase Chain Reactions (PCRs), considering as criteria oligonucleotide melting temperature, size, GC content and primer-dimer possibilities, PCR product size, positional constraints within the source sequence, and miscellaneous other constraints. All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.

This package contains example data to test the package.

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Homepage

http://primer3.sourceforge.net


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