trace2dbest

Description

ESTs are short sequences derived from reverse-transcribed RNA. Their abundances yield insights in the expression of genes across tissues, support the discovery of new genes and allow one to assess the coverage of whole genome sequencing projects. Public databases like dbEST at the NCBI collect this data.

trace2dbEST process raw sequenceing chromatograph trace files from EST projects into quality-checked sequences, ready for submission to dbEST. trace2dbEST guides you through the creation of all the necessary files for submission of ESTs to dbEST. trace2dbest makes use of other software (available free under academic licence) that you will need to have installed, namely phred, cross_match and (optionally) BLAST.

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Homepage

http://www.nematodes.org/bioinformatics/trace2dbEST/

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