ChimeraSlayer is a chimeric sequence detection utility, compatible with
near-full length Sanger sequences and shorter 454-FLX sequences (~500bp).
Chimera Slayer involves the following series of steps that operate to
flag chimeric 16S rRNA sequences:
1. the ends of a query sequence are searched against an included
database of reference chimera-free 16S sequences to identify potential
parents of a chimera
2. candidate parents of a chimera are selected as those that form a
branched best scoring alignment to the NAST-formatted query sequence
3. the NAST alignment of the query sequence is improved in a
‘chimera-aware’ profile-based NAST realignment to the selected
reference parent sequences
4. an evolutionary framework is used to flag query sequences found to
exhibit greater sequence homology to an in silico chimera formed
between any two of the selected reference parent sequences.
To run Chimera Slayer, you need NAST-formatted sequences generated by
the nast-ier utility.
ChimeraSlayer is part of the microbiomeutil suite.
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Install this software package
If the package is available for the distribution you are currently using on your computer then install the software by clicking on… Install chimeraslayer