To facilitate the multiple phases of the dazzler assembler, all the read data
is organized into what is effectively a database of the
reads and their meta-information. The design goals for this data base
are as follows:
* The database stores the source Pacbio read information in such a
way that it can re-create the original input data, thus permitting
a user to remove the (effectively redundant) source files. This
avoids duplicating the same data, once in the source file and once
in the database.
* The data base can be built up incrementally, that is new sequence
data can be added to the data base over time.
* The data base flexibly allows one to store any meta-data desired for
reads. This is accomplished with the concept of *tracks* that
implementors can add as they need them.
* The data is held in a compressed form equivalent to the .dexta and
.dexqv files of the data extraction module. Both the .fasta and
.quiva information for each read is held in the data base and can be
recreated from it. The .quiva information can be added separately and
later on if desired.
* To facilitate job parallel, cluster operation of the phases of the
assembler, the database has a concept of a *current partitioning* in
which all the reads that are over a given length and optionally
unique to a well, are divided up into *blocks* containing roughly a
given number of bases, except possibly the last block which may have
a short count. Often programs can be run on blocks or pairs of blocks
and each such job is reasonably well balanced as the blocks are all
the same size. One must be careful about changing the partition
during an assembly as doing so can void the structural validity of
any interim block-based results.
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Install this software package
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