mosdepth
BAM/CRAM depth calculation biological sequencing
Description
Many small reads are produced by high-throughput "next generation" sequencing technologies. The final sequence is derived from how these reads are overlapping towards a consensus. The more reads are covering/confirming parts of a nucleotide seq, the higher the confidence is. Too many reads would be indicative of e.g. repeats in the genome. mosdepth can output: * per-base depth about 2x as fast samtools depth--about 25 minutes
of CPU time for a 30X genome.
* mean per-window depth given a window size--as would be used for
CNV calling.
* the mean per-region given a BED file of regions.
* a distribution of proportion of bases covered at or above a given
threshold for each chromosome and genome-wide.
* quantized output that merges adjacent bases as long as they fall
in the same coverage bins e.g. (10-20)
* threshold output to indicate how many bases in each region are
covered at the given thresholds.
when appropriate, the output files are bgzipped and indexed for ease
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Homepage
https://github.com/brentp/mosdepth
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