Run conditional reciprocal best blast
DescriptionCRB-BLAST is a novel method for finding orthologs between one set of sequences and another. This is particularly useful in genome and transcriptome annotation. CRB-BLAST initially performs a standard reciprocal best BLAST. It does this by performing BLAST alignments of query->target and target->query. Reciprocal best BLAST hits are those where the best match for any given query sequence in the query->target alignment is also the best hit of the match in the reverse (target->query) alignment. Reciprocal best BLAST is a very conservative way to assign orthologs. The main innovation in CRB-BLAST is to learn an appropriate e-value cutoff to apply to each pairwise alignment by taking into account the overall relatedness of the two datasets being compared. This is done by fitting a function to the distribution of alignment e-values over sequence lengths. The function provides the e-value cutoff for a sequence of given length.
Upload more screenshots
Please help extend the collection of screenshots. Just make a screenshot and upload it here. You don't need to register or anything.Upload a screenshot
Hint: upload an image here from your clipboard with Ctrl-V
Install this software package
If the package is available for the distribution you are currently using on your computer then install the software by clicking on…Install ruby-crb-blast