trimmomatic
flexible read trimming tool for Illumina NGS data
Description
Trimmomatic performs a variety of useful trimming tasks for illumina paired-end and single ended data.The selection of trimming steps and their associated parameters are supplied on the command line. The current trimming steps are: * ILLUMINACLIP: Cut adapter and other illumina-specific sequences from
the read.
* SLIDINGWINDOW: Perform a sliding window trimming, cutting once thes
average quality within the window falls below a threshold.
* LEADING: Cut bases off the start of a read, if below a threshold quality
* TRAILING: Cut bases off the end of a read, if below a threshold quality
* CROP: Cut the read to a specified length
* HEADCROP: Cut the specified number of bases from the start of the read
* MINLENGTH: Drop the read if it is below a specified length
* TOPHRED33: Convert quality scores to Phred-33
* TOPHRED64: Convert quality scores to Phred-64
It works with FASTQ (using phred + 33 or phred + 64 quality scores,
depending on the Illumina pipeline used), either uncompressed or
gzipp'ed FASTQ. Use of gzip format is determined based on the .gz
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Homepage
http://www.usadellab.org/cms/index.php?page=trimmomatic
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